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mouse anti cd80 66406  (Proteintech)


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    Structured Review

    Proteintech mouse anti cd80 66406
    Mouse Anti Cd80 66406, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cd80+66406/Histone+H4+Antibody/pmc12915221-250-55-54
    Average 97 stars, based on 145 article reviews
    mouse anti cd80 66406 - by Bioz Stars, 2026-10
    97/100 stars

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    Incubation:

    Article Title: Fmoc-DDT@Fos hydrogel mitigates temporomandibular joint osteoarthritis through regulating macrophage reprogramming and ferroptosis
    Article Snippet: Ferroptosis was induced by Treating with 5 μM Erastin (HY-15763, MedChemExpress, USA). .. Samples were fixed in 4% paraformaldehyde (PFA), blocked with 5% BSA, and incubated overnight at 4 °C with primary antibodies from Abcam (rabbit anti-SYK, ab155187, 1:500; rabbit anti-CD206, ab64693, 1:500; rabbit anti-CD45, ab281586, 1:50), Thermo Fisher (rabbit anti-CD86, 942-RBM4-P1, 1:500; rabbit anti-Collagen II, PA5-99159, 1:100; rabbit anti-ACSL4, MA5-42756, 1:100; mouse anti-GPX4, 2879-MSM1-P0, 1:100), and Proteintech (mouse anti-CD80, 66406, 1:100). .. After PBS rinsing, Alexa Fluor 594 (ab150116, 1:200, Abcam) or Alexa Fluor 488 (ab150077, 1:200, Abcam) secondary antibodies were applied for 1 h at RT, followed by DAPI staining (C1002, Beyotime) for 10 min.



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    Proteintech mouse anti cd80 66406
    Mouse Anti Cd80 66406, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cd80+66406/Histone+H4+Antibody/pmc12915221-250-55-54
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    Proteintech mouse anti cd80 monoclonal primary antibody
    Mouse Anti Cd80 Monoclonal Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech anti mouse cd80
    a RAW 264.7 cells were polarized into M0, M1 (induced by IFN-γ and LPS), or M2 (induced by IL-4 and IL-13) macrophages. M0 macrophages were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Macrophages were stained with ActinRed (red), and nuclei were stained with Hoechst (blue). b Polarized M0 RAW 264.7 cells were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Flow cytometry analysis of CD86 and CD206 expression was performed. c , d RNA-sequencing analyses of polarized M2 RAW 264.7 cells treated with or without 1 Hz RMF for 15 min. KEGG enrichment analysis and heat map analysis were performed ( n = 3 independent biological replicates). e , f RAW 264.7 cells or BMDMs were polarized into M0, M1, or M2 macrophages. M2 macrophages were incubated with MNMs and then treated with or without 1 Hz RMF for 15 min (M2-MNM represents M2 macrophages incubated with MNMs, and M2-RMF represents M2 macrophages incubated with MNMs and treated with RMF). Concentrations of M1-associated proteins (IL-1β) and M2-associated proteins (IL-10) were examined in the conditioned medium of these cells. Data are presented as means ± SD. Statistical significance was defined as P < 0.05 ( n = 3 independent biological replicates). g RAW 264.7 cells were polarized into M0, M1, or M2 macrophages. M0 macrophages were cultured in conditioned medium derived from LLC and incubated with or without MNMs, then treated with or without 1 Hz RMF for 15 min. mRNA levels of M1-associated genes ( <t>Cd80</t> , Cd86 , Il1b , Il6 ) and M2-associated genes ( Cd163 , Cd206 , Il4 , Il10 ) were examined. Data are presented as means ± SD ( n = 4 independent biological replicates).
    Anti Mouse Cd80, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech anti cd80 mouse 66406 1 ig
    a RAW 264.7 cells were polarized into M0, M1 (induced by IFN-γ and LPS), or M2 (induced by IL-4 and IL-13) macrophages. M0 macrophages were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Macrophages were stained with ActinRed (red), and nuclei were stained with Hoechst (blue). b Polarized M0 RAW 264.7 cells were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Flow cytometry analysis of CD86 and CD206 expression was performed. c , d RNA-sequencing analyses of polarized M2 RAW 264.7 cells treated with or without 1 Hz RMF for 15 min. KEGG enrichment analysis and heat map analysis were performed ( n = 3 independent biological replicates). e , f RAW 264.7 cells or BMDMs were polarized into M0, M1, or M2 macrophages. M2 macrophages were incubated with MNMs and then treated with or without 1 Hz RMF for 15 min (M2-MNM represents M2 macrophages incubated with MNMs, and M2-RMF represents M2 macrophages incubated with MNMs and treated with RMF). Concentrations of M1-associated proteins (IL-1β) and M2-associated proteins (IL-10) were examined in the conditioned medium of these cells. Data are presented as means ± SD. Statistical significance was defined as P < 0.05 ( n = 3 independent biological replicates). g RAW 264.7 cells were polarized into M0, M1, or M2 macrophages. M0 macrophages were cultured in conditioned medium derived from LLC and incubated with or without MNMs, then treated with or without 1 Hz RMF for 15 min. mRNA levels of M1-associated genes ( <t>Cd80</t> , Cd86 , Il1b , Il6 ) and M2-associated genes ( Cd163 , Cd206 , Il4 , Il10 ) were examined. Data are presented as means ± SD ( n = 4 independent biological replicates).
    Anti Cd80 Mouse 66406 1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech anti mouse cd80 mab
    a RAW 264.7 cells were polarized into M0, M1 (induced by IFN-γ and LPS), or M2 (induced by IL-4 and IL-13) macrophages. M0 macrophages were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Macrophages were stained with ActinRed (red), and nuclei were stained with Hoechst (blue). b Polarized M0 RAW 264.7 cells were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Flow cytometry analysis of CD86 and CD206 expression was performed. c , d RNA-sequencing analyses of polarized M2 RAW 264.7 cells treated with or without 1 Hz RMF for 15 min. KEGG enrichment analysis and heat map analysis were performed ( n = 3 independent biological replicates). e , f RAW 264.7 cells or BMDMs were polarized into M0, M1, or M2 macrophages. M2 macrophages were incubated with MNMs and then treated with or without 1 Hz RMF for 15 min (M2-MNM represents M2 macrophages incubated with MNMs, and M2-RMF represents M2 macrophages incubated with MNMs and treated with RMF). Concentrations of M1-associated proteins (IL-1β) and M2-associated proteins (IL-10) were examined in the conditioned medium of these cells. Data are presented as means ± SD. Statistical significance was defined as P < 0.05 ( n = 3 independent biological replicates). g RAW 264.7 cells were polarized into M0, M1, or M2 macrophages. M0 macrophages were cultured in conditioned medium derived from LLC and incubated with or without MNMs, then treated with or without 1 Hz RMF for 15 min. mRNA levels of M1-associated genes ( <t>Cd80</t> , Cd86 , Il1b , Il6 ) and M2-associated genes ( Cd163 , Cd206 , Il4 , Il10 ) were examined. Data are presented as means ± SD ( n = 4 independent biological replicates).
    Anti Mouse Cd80 Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    Proteintech anti mouse cd80 antibody
    a RAW 264.7 cells were polarized into M0, M1 (induced by IFN-γ and LPS), or M2 (induced by IL-4 and IL-13) macrophages. M0 macrophages were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Macrophages were stained with ActinRed (red), and nuclei were stained with Hoechst (blue). b Polarized M0 RAW 264.7 cells were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Flow cytometry analysis of CD86 and CD206 expression was performed. c , d RNA-sequencing analyses of polarized M2 RAW 264.7 cells treated with or without 1 Hz RMF for 15 min. KEGG enrichment analysis and heat map analysis were performed ( n = 3 independent biological replicates). e , f RAW 264.7 cells or BMDMs were polarized into M0, M1, or M2 macrophages. M2 macrophages were incubated with MNMs and then treated with or without 1 Hz RMF for 15 min (M2-MNM represents M2 macrophages incubated with MNMs, and M2-RMF represents M2 macrophages incubated with MNMs and treated with RMF). Concentrations of M1-associated proteins (IL-1β) and M2-associated proteins (IL-10) were examined in the conditioned medium of these cells. Data are presented as means ± SD. Statistical significance was defined as P < 0.05 ( n = 3 independent biological replicates). g RAW 264.7 cells were polarized into M0, M1, or M2 macrophages. M0 macrophages were cultured in conditioned medium derived from LLC and incubated with or without MNMs, then treated with or without 1 Hz RMF for 15 min. mRNA levels of M1-associated genes ( <t>Cd80</t> , Cd86 , Il1b , Il6 ) and M2-associated genes ( Cd163 , Cd206 , Il4 , Il10 ) were examined. Data are presented as means ± SD ( n = 4 independent biological replicates).
    Anti Mouse Cd80 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cd80+66406/CD80%2FB7-1+Antibody/pm32771520-71-22-26
    Average 96 stars, based on 1 article reviews
    anti mouse cd80 antibody - by Bioz Stars, 2026-10
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    Proteintech mouse anti cd80 antibody
    a RAW 264.7 cells were polarized into M0, M1 (induced by IFN-γ and LPS), or M2 (induced by IL-4 and IL-13) macrophages. M0 macrophages were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Macrophages were stained with ActinRed (red), and nuclei were stained with Hoechst (blue). b Polarized M0 RAW 264.7 cells were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Flow cytometry analysis of CD86 and CD206 expression was performed. c , d RNA-sequencing analyses of polarized M2 RAW 264.7 cells treated with or without 1 Hz RMF for 15 min. KEGG enrichment analysis and heat map analysis were performed ( n = 3 independent biological replicates). e , f RAW 264.7 cells or BMDMs were polarized into M0, M1, or M2 macrophages. M2 macrophages were incubated with MNMs and then treated with or without 1 Hz RMF for 15 min (M2-MNM represents M2 macrophages incubated with MNMs, and M2-RMF represents M2 macrophages incubated with MNMs and treated with RMF). Concentrations of M1-associated proteins (IL-1β) and M2-associated proteins (IL-10) were examined in the conditioned medium of these cells. Data are presented as means ± SD. Statistical significance was defined as P < 0.05 ( n = 3 independent biological replicates). g RAW 264.7 cells were polarized into M0, M1, or M2 macrophages. M0 macrophages were cultured in conditioned medium derived from LLC and incubated with or without MNMs, then treated with or without 1 Hz RMF for 15 min. mRNA levels of M1-associated genes ( <t>Cd80</t> , Cd86 , Il1b , Il6 ) and M2-associated genes ( Cd163 , Cd206 , Il4 , Il10 ) were examined. Data are presented as means ± SD ( n = 4 independent biological replicates).
    Mouse Anti Cd80 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cd80+66406/CD80%2FB7-1+Antibody/pm30902794-69-10-14
    Average 96 stars, based on 1 article reviews
    mouse anti cd80 antibody - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    a RAW 264.7 cells were polarized into M0, M1 (induced by IFN-γ and LPS), or M2 (induced by IL-4 and IL-13) macrophages. M0 macrophages were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Macrophages were stained with ActinRed (red), and nuclei were stained with Hoechst (blue). b Polarized M0 RAW 264.7 cells were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Flow cytometry analysis of CD86 and CD206 expression was performed. c , d RNA-sequencing analyses of polarized M2 RAW 264.7 cells treated with or without 1 Hz RMF for 15 min. KEGG enrichment analysis and heat map analysis were performed ( n = 3 independent biological replicates). e , f RAW 264.7 cells or BMDMs were polarized into M0, M1, or M2 macrophages. M2 macrophages were incubated with MNMs and then treated with or without 1 Hz RMF for 15 min (M2-MNM represents M2 macrophages incubated with MNMs, and M2-RMF represents M2 macrophages incubated with MNMs and treated with RMF). Concentrations of M1-associated proteins (IL-1β) and M2-associated proteins (IL-10) were examined in the conditioned medium of these cells. Data are presented as means ± SD. Statistical significance was defined as P < 0.05 ( n = 3 independent biological replicates). g RAW 264.7 cells were polarized into M0, M1, or M2 macrophages. M0 macrophages were cultured in conditioned medium derived from LLC and incubated with or without MNMs, then treated with or without 1 Hz RMF for 15 min. mRNA levels of M1-associated genes ( Cd80 , Cd86 , Il1b , Il6 ) and M2-associated genes ( Cd163 , Cd206 , Il4 , Il10 ) were examined. Data are presented as means ± SD ( n = 4 independent biological replicates).

    Journal: Cell Research

    Article Title: Dynamic magneto-mechanical force in lysosomes induces durable macrophage repolarization for antitumor immunity

    doi: 10.1038/s41422-025-01217-1

    Figure Lengend Snippet: a RAW 264.7 cells were polarized into M0, M1 (induced by IFN-γ and LPS), or M2 (induced by IL-4 and IL-13) macrophages. M0 macrophages were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Macrophages were stained with ActinRed (red), and nuclei were stained with Hoechst (blue). b Polarized M0 RAW 264.7 cells were incubated with MNMs and then treated with 1 Hz RMF for 15 min. Flow cytometry analysis of CD86 and CD206 expression was performed. c , d RNA-sequencing analyses of polarized M2 RAW 264.7 cells treated with or without 1 Hz RMF for 15 min. KEGG enrichment analysis and heat map analysis were performed ( n = 3 independent biological replicates). e , f RAW 264.7 cells or BMDMs were polarized into M0, M1, or M2 macrophages. M2 macrophages were incubated with MNMs and then treated with or without 1 Hz RMF for 15 min (M2-MNM represents M2 macrophages incubated with MNMs, and M2-RMF represents M2 macrophages incubated with MNMs and treated with RMF). Concentrations of M1-associated proteins (IL-1β) and M2-associated proteins (IL-10) were examined in the conditioned medium of these cells. Data are presented as means ± SD. Statistical significance was defined as P < 0.05 ( n = 3 independent biological replicates). g RAW 264.7 cells were polarized into M0, M1, or M2 macrophages. M0 macrophages were cultured in conditioned medium derived from LLC and incubated with or without MNMs, then treated with or without 1 Hz RMF for 15 min. mRNA levels of M1-associated genes ( Cd80 , Cd86 , Il1b , Il6 ) and M2-associated genes ( Cd163 , Cd206 , Il4 , Il10 ) were examined. Data are presented as means ± SD ( n = 4 independent biological replicates).

    Article Snippet: To explore the proportions of different macrophage subtypes in tumor tissues after MagLMP treatment, macrophages in the sections were stained by immunofluorescence with anti-mouse CD206 (R&D Systems, AF2535), anti-mouse CD80 (Proteintech, 14292-1-AP), anti-mouse F4/80 (R&D Systems, MAB5580), and DAPI (Beyotime Biotech, C1002).

    Techniques: Incubation, Staining, Flow Cytometry, Expressing, RNA Sequencing, Cell Culture, Derivative Assay